Journal: Frontiers in Immunology

Article Title: Novel biomarkers for prediction of atonic postpartum hemorrhage among ‘low-risk’ women in labor

doi: 10.3389/fimmu.2024.1416990

Figure Lengend Snippet: (A–E) Violin plots show significant differences in 5 plasma cytokines between the atonic PPH group and the normal control group, including (A) IL-2rα , (B) IL-9 , (C) TNF-β , (D) MIP1β and (E) CTACK . IL-2rα , interleukin−2Rα; IL-9 , interleukin−9; TNF-β , tumor necrosis factor−β; MIP1β , macrophage inflammatory protein-1β ; CTACK , cutaneous T cell-attracting chemokine. Mann-Whitney U test was used for assessing intergroup differences across these factors. * P<0.05; ** P<0.01; *** P<0.001 .

Article Snippet: Human IL2Rα, IL9, MIP1β, TNFβ, and CTACK ELISA kits from Thermo Fisher Scientific (Waltham, MA, USA) were employed for prospective temporal validation.

Techniques: Clinical Proteomics, Control, MANN-WHITNEY

Journal: Frontiers in Immunology

Article Title: Novel biomarkers for prediction of atonic postpartum hemorrhage among ‘low-risk’ women in labor

doi: 10.3389/fimmu.2024.1416990

Figure Lengend Snippet: Predictor selection using LASSO regression analysis with tenfold cross-validation. (A) LASSO coefficient profiles of the 14 risk factors were created against the log ( λ ) sequence. (B) Tuning parameter (lambda, λ ) selection of deviance in the LASSO regression based on the minimum criterion (left dotted line) and the 1-SE criterion (right dotted line). In the present study, predictor selection was performed according to the minimum criterion (including Prenatal Hb, Lymph%, PLR, LnSII, IL2Rα, MIP1β, TNFβ, CTACK , and IL9 ). LASSO least absolute shrinkage and selection operator, SE standard error.

Article Snippet: Human IL2Rα, IL9, MIP1β, TNFβ, and CTACK ELISA kits from Thermo Fisher Scientific (Waltham, MA, USA) were employed for prospective temporal validation.

Techniques: Selection, Biomarker Discovery, Sequencing

Journal: Frontiers in Immunology

Article Title: Novel biomarkers for prediction of atonic postpartum hemorrhage among ‘low-risk’ women in labor

doi: 10.3389/fimmu.2024.1416990

Figure Lengend Snippet: (A–I) ROC curves of identified biomarkers for atonic PPH: (A) IL−2Rα (AUC: 715, 95% CI 0.600–0.829, P < 0.001), (B) IL−9 (AUC: 0.677, 95% CI 0.554–0.800, P = 0.003), (C) TNF−β (AUC: 0.657, 95% CI 0.535–0.764, P = 0.017), (D) MIP1β(AUC: 0.626, 95% CI 0.501–0.752, P = 0.026), (E) CTACK (AUC: 0.638, 95% CI 0.513–0.767, P = 0.039), (F) Prenatal Hb (AUC: 0.637, 95% CI 0.514–0.760, P = 0.017), (G) Lymph% (AUC: 0.688, 95% CI 0.568–0.807, P = 0.002), (H) PLR (AUC: 0.641, 95% CI 0.519–0.762, P = 0.015), and (I) LnSII (AUC: 0.705, 95% CI 0.589–0.821, P = 0.001). IL2Rα , interleukin-2 receptor subunit α; MIP1β , macrophage inflammatory protein-1β; TNF-β , tumor necrosis factor-β; IL9 , interleukin-9; CTACK , cutaneous T cell-attracting chemokine; Hb , hemoglobin; Lymph% , lymphocyte ratio; PLR , platelet-to-lymphocyte ratio; SII , systemic immune-inflammation index; LnSII , natural logarithm of SII; ROC, receiver operating characteristic; AUC, area under the ROC curve.

Article Snippet: Human IL2Rα, IL9, MIP1β, TNFβ, and CTACK ELISA kits from Thermo Fisher Scientific (Waltham, MA, USA) were employed for prospective temporal validation.

Techniques:

Journal: Frontiers in Immunology

Article Title: Novel biomarkers for prediction of atonic postpartum hemorrhage among ‘low-risk’ women in labor

doi: 10.3389/fimmu.2024.1416990

Figure Lengend Snippet: Nomogram1 and nomogram2 were constructed to predict the incidence of atonic PPH among women in labor. (A) Nomogram1 including IL2Rα, IL9, MIP1β, TNF-β, CTACK, Prenatal Hb, Lymph%, PLR, and LnSII for assessing the risk of atonic PPH among women in labor. (B) Nomogram2 including IL2Rα, IL9, Prenatal Hb, and PLR for assessing the risk of atonic PPH among women in labor. Nomogram1 and nomogram2 are used to obtain the risk of atonic PPH by adding up the points identified on the points’ scale for each variable. IL2Rα , interleukin-2 receptor subunit α; MIP1β , macrophage inflammatory protein-1β; TNF-β , tumor necrosis factor-β; IL9 , interleukin-9; CTACK , cutaneous T cell-attracting chemokine; Hb , hemoglobin; Lymph% , lymphocyte ratio; PLR , platelet-to-lymphocyte ratio; SII , systemic immune-inflammation index; LnSII , natural logarithm of SII.

Article Snippet: Human IL2Rα, IL9, MIP1β, TNFβ, and CTACK ELISA kits from Thermo Fisher Scientific (Waltham, MA, USA) were employed for prospective temporal validation.

Techniques: Construct

Journal: Frontiers in Immunology

Article Title: Novel biomarkers for prediction of atonic postpartum hemorrhage among ‘low-risk’ women in labor

doi: 10.3389/fimmu.2024.1416990

Figure Lengend Snippet: Validation of differentially expressed markers in a prospective cohort.

Article Snippet: Human IL2Rα, IL9, MIP1β, TNFβ, and CTACK ELISA kits from Thermo Fisher Scientific (Waltham, MA, USA) were employed for prospective temporal validation.

Techniques: Biomarker Discovery, Control

Journal: Frontiers in Immunology

Article Title: Novel biomarkers for prediction of atonic postpartum hemorrhage among ‘low-risk’ women in labor

doi: 10.3389/fimmu.2024.1416990

Figure Lengend Snippet: (A–I) Violin plots illustrate validated differentially expressed biomarkers between the atonic PPH group and control group in a prospective cohort, including (A) IL-2rα , (B) IL-9 , (C) TNF-β , (D) MIP1β , (E) CTACK , (F) Prenatal Hb , (G) Lymph% , (H) PLR and (I) LnSII. IL-2rα , interleukin−2Rα; IL-9 , interleukin−9; TNF-β , tumor necrosis factor−β; MIP1β , macrophage inflammatory protein-1β; CTACK , cutaneous T cell-attracting chemokine; Hb , hemoglobin; Lymph% , proportion of lymphocytes; PLR , platelet-to-lymphocyte ratio; LnSII , natural logarithm of SII. Data are presented as mean ± standard deviation or median (interquartile range). Independent samples t-test was employed for Prenatal Hb and LnSII . Mann-Whitney U test was used for IL-2rα , IL-9 , TNF-β , MIP1β , CTACK , Lymph% and PLR. * P <0.05; ** P <0.01; *** P <0.001; **** P <0.0001.

Article Snippet: Human IL2Rα, IL9, MIP1β, TNFβ, and CTACK ELISA kits from Thermo Fisher Scientific (Waltham, MA, USA) were employed for prospective temporal validation.

Techniques: Control, Standard Deviation, MANN-WHITNEY

Journal: Mucosal immunology

Article Title: Duplication of the IL2RA locus causes excessive IL-2 signaling and may predispose to very early onset colitis.

doi: 10.1038/s41385-021-00423-5

Figure Lengend Snippet: Fig. 1 A 374 kb duplication on 10p15.1 including the IL2RA locus leads to intrinsically increased CD25 and enhanced IL-2 signaling in CD4+ T cells. a Timeline depicting the patient’s clinical course and time points of sample collection (denoted as visits S0-S4). PUCAI, pediatric ulcerative colitis activity index. b H&E staining on paraffin-embedded colonic tissue at the time of diagnosis (left, time point S0) and immunohistochemical detection of CD4 and CD8 in paraffin-embedded resected colonic tissue (right, time point S2). c Flow cytometric analysis of CD4:CD8 ratio in peripheral blood of the patient, her parents (time points S3 and S4), and VEO-IBD patients (n = 9). Median (5th to 95th percentiles) for the 2–5 year age category is 1.6 (0.9–2.9).23 d Localization of the patient’s duplication on chromosome 10. e–g Flow cytometric analysis of CD3, CD4, CD38, CD62L, CD45RA, CCR7, CD25, and/or Foxp3 expression was performed on peripheral blood from the patient, her parents (time points S3 and S4) and healthy adult controls (HC, n = 4–6). e CD25 expression (MFI) on total CD4+ T cells (left) and on effector memory (CD45RAnegCCR7neg), central memory (CD45RAnegCCR7+) and naive (CD45RA+CCR7+) CD4+ T cells in the patient (right). f CD25 expression on regulatory Foxp3+CD4+ T cells and Foxp3negCD4+ T cells. g Frequency of pSTAT5-positive cells among control primary T cells transduced with retrovirus particles carrying full-length IL2RA or an empty vector upon stimulation with increasing concentrations of IL- 2 (0.2 to 100IU/mL) (n = 4, p = 0.0143 at 0.2 IU/mL was calculated using Wilcoxon-Mann Whitney test). h PBMCs of the patient, PIBD patients with active intestinal inflammation (n = 3), and PIBD patients in remission (n = 3) were stimulated with IL-2 (100 IU/mL) for 15 min followed by quantification of STAT5 phosphorylation (pY694) in CD4+ cells by flow cytometry (visit S3). n.s., not significant, *p < 0.05, **p < 0.01, ***p < 0.001 using one-way ANOVA followed by the Bonferroni’s Multiple Comparison Test.

Article Snippet: Plasmids, retrovirus production, transduction, and pSTAT5 activation The IL2RA cDNA was subcloned from pEGFP-N1 (#86055, Addgene, Watertown, MA, USA) into a pLZRS-IRES-ΔNGFR (#72930, Addgene) via Dra1/EcoR1 sites and the tag EGFP was cut-out.

Techniques: Activity Assay, Staining, Biomarker Discovery, Immunohistochemical staining, Expressing, Control, Transduction, Plasmid Preparation, MANN-WHITNEY, Phospho-proteomics, Cytometry, Comparison

Journal: Mucosal immunology

Article Title: Duplication of the IL2RA locus causes excessive IL-2 signaling and may predispose to very early onset colitis.

doi: 10.1038/s41385-021-00423-5

Figure Lengend Snippet: Fig. 3 Increased proliferation and activation of colonic CD4+ IEL and LPL in the patient with IL2RA duplication compared to treatment- resistant pediatric-onset IBD. a, f, h Representative immunohistochemical staining for CD3, pSTAT5, and Tbet in paraffin-embedded resected inflamed colonic tissue (visit S2) and paraffin-embedded tissue of the unaffected duodenum from the patient at time of diagnosis (visit S0) and PIBD control. b CD3, CD4, and CD8 mRNA and g IFNG mRNA expression in total resected colonic tissue of the patient and PIBD controls. c CD3, CD4, and CD8 mRNA and (i) HLAE mRNA expression in the epithelial layer isolated from resected colonic tissue of the patient and PIBD controls. d Representative immunofluorescent double staining of paraffin-embedded resected colonic tissue of the patient. Green = Ki67, red = CD3, blue = 4′,6-diamidino-2-phenylindole (DAPI) nuclear staining. e LPL were isolated from the inflamed colonic tissue of the patient. Frequencies of CD4+ and CD8+ cells in live CD3+ LPL, and Ki67 and CD25 expression by CD4+ and CD8+ LPL were analyzed by flow cytometry.

Article Snippet: Plasmids, retrovirus production, transduction, and pSTAT5 activation The IL2RA cDNA was subcloned from pEGFP-N1 (#86055, Addgene, Watertown, MA, USA) into a pLZRS-IRES-ΔNGFR (#72930, Addgene) via Dra1/EcoR1 sites and the tag EGFP was cut-out.

Techniques: Activation Assay, Immunohistochemical staining, Staining, Biomarker Discovery, Control, Expressing, Isolation, Double Staining, Cytometry